Por título Por autor Por editorial

búsqueda avanzada

 Synthetic messenger RNA and cell metabolism modulation

Synthetic messenger RNA and cell metabolism modulation

Autor:

Editorial:
Springer (Alemania)

Año de edición:

ISBN:
978-1-62703-259-9

Páginas:
370

AR$ 14725.00
Agregar al carrito
Este producto está disponible en Alemania y a través de CastellanoLibros.com usted lo recibirá en su domicilio.

Calcular el costo de envío

Descripción

Methods and Protocols. Series: Methods in Molecular Biology, Vol. 969. Hardcover.

Más detalles ↓

Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

Synthetic mRNA is an attractive tool for mammalian cell reprogramming that  can be used in basic research, as well as in clinical applications. Present mRNA in vitro synthesis is a rather simple procedure, which delivers a high yield of quality product. Various modifications may be introduced into the mRNA by changing  the sequence of the DNA template, by modifying the reaction of transcription, or by post-transcriptional modification. mRNA, as a transfection agent, has several advantages over DNA, as mRNA expression is not dependent on nuclear entry and occurs directly in the cytosol. Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols covers the typical main methods, such as mRNA synthesis, modifications, and delivery.  Examples of cell reprogramming and analysis in the fields of immunotherapy and stem cell research are also included. Written in the successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.
 
Authoritative and easily accessible, Synthetic Messenger RNA and Cell Metabolism Modulation: Methods and Protocols will be of interest to researchers, clinicians, and biotech companies interested in mRNA-mediated cell reprogramming.
Content Level » Professional/practitioner
Keywords » cell metabolism - cell reprogramming - immunotherapy - mRNA synthesis - synthetic messenger RNA - transfection agent
Related subjects » Human Genetics
TABLE OF CONTENTS
Part I. mRNA Synthesis, Purification, and Transfer
 
1. Cell Engineering with Synthetic Messenger RNA 
            Peter M. Rabinovich and Sherman M. Weissman
 
2. In vitro Transcription of Long RNA Containing Modified Nucleosides
Norbert Pardi, Hiromi Muramatsu, Drew Weissman, and Katalin Karikó
 
3. HPLC Purification of in vitro Transcribed Long RNA
            Drew Weissman, Norbert Pardi, Hiro Muramatsu, and Katalin Karikó
 
4. Synthetic mRNAs with Superior Translation and Stability Properties
            Ewa Grudzien-Nogalska, Joanna Kowalska, Wei Su, Andreas N. Kuhn, Sergey V. Slepenkov, Edward Darzynkiewicz, Ugur Sahin, Jacek Jemielity, and Robert E. Rhoads
 
5. Non-Viral, Cationic Lipid-Mediated Delivery of mRNA 
            James G. Hecker
 
6. Light-Induced mRNA Transfection  
            Sigurd Leinæs Bøe and Eivind Hovig
 
7. Engineering B Cells with mRNA 
            Jaewoo Lee, David Boczkowski, and Smita Nair
 
8. Programming Human Dendritic Cells with mRNA
            Jaewoo Lee, David Boczkowski, and Smita Nair
 
9. Large Volume Flow Electroporation of mRNA: Clinical Scale Process
            Linhong Li, Cornell Allen, Rama Shivakumar, and Madhusudan V. Peshwa
 
10. Retrovirus-Based mRNA Transfer for Transient Cell Manipulation
            Melanie Galla, Axel Schambach, and Christopher Baum
 
11. mRNA Delivery to Human Dendritic Cells by Recombinant Yeast and Activation of Antigen-Specific Memory T Cells
            Frank Breinig, Tanja Breinig, and Manfred J. Schmitt
 
 
Part 2. Modulation and monitoring of cell metabolism      
 
12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy
            Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken
 
13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors
            Noriko Shimasaki and Dario Campana
 
14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA
            Peizhe Wang and Jie Na
 
15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies
            Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin
 
16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA
            Chantal Pichon and Patrick Midoux
 
17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA
            Özcan Met and Inge Marie Svane
 
18. mRNA electroporation as a Tool for Immunomonitoring
            Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman
 
19. mRNA PCR-Based Epitope Chase Method          
            Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe
 
 
Part 2. Modulation and monitoring of cell metabolism      
 
12. Non-Viral RNA Transfection to Transiently Modify T Cells with Chimeric Antigen Receptors for Adoptive Therapy
            Tobias Riet, Astrid Holzinger, Jan Dörrie, Niels Schaft, Gerold Schuler, and Hinrich Abken
 
13. Natural Killer Cell Reprogramming with Chimeric Immune Receptors
            Noriko Shimasaki and Dario Campana
 
14. Reprogramming to Pluripotency and Differentiation of Cells with Synthetic mRNA
            Peizhe Wang and Jie Na
 
15. Anti-Tumor Vaccination with Synthetic mRNA: Strategies for in vitro and in vivo Preclinical Studies
            Mustafa Diken, Sebastian Kreiter, Abderraouf Selmi, Özlem Türeci, and Ugur Sahin
 
16. Mannosylated and Histidylated LPR Technology for Vaccination with Tumor Antigen mRNA
            Chantal Pichon and Patrick Midoux
 
17. Analysis of Surviving-Specific T Cells in Breast Cancer Patients Using Human DCs Engineered with Survivin mRNA
            Özcan Met and Inge Marie Svane
 
18. mRNA electroporation as a Tool for Immunomonitoring
            Nathalie Cools, Kirsten Van Camp, Viggo Van Tendeloo, and Zwi Berneman
 
19. mRNA PCR-Based Epitope Chase Method          
            Jean-Daniel Doucet, Dominique Gauchat, and Réjean Lapointe
 

Catalogado en

Importados / Medicina - Genética

Libros relacionados
How to be Profitable and Moral

How to be Profitable and Moral

Woiceshyn, Jaana

AR$ 3040.00

Agregar al carro

El mercado de trabajo en la obra de Luis Toharia

El mercado de trabajo en la obra de Luis...

Toharia Cortés, Luis

AR$ 3087.50

Agregar al carro

Dead firms

Dead firms

González-Pérez, Mª Alejandra - Cathro, V...

AR$ 17575.00

Agregar al carro

Manual Práctico sobre la Nueva Contabilidad Local

Manual Práctico sobre la Nueva Contabili...

Aragón Sánchez, Fracesc

AR$ 7505.00

Agregar al carro